Publications

Pradhan K, Neupane B, Niehues P, Kirschner M, Beier F, Kuo C-C, Hilbold E, Bär C, Thoma O-M, Waldner M, Vieri M, Brümmendorf T, Tharmapalan V, Wagner W, Kleines M, Emrani M, Zink M, Napp A, Marx N, and Gramlich M. Telomere length is associated with adverse atrial remodeling in patients with atrial fibrillation. Journal of the American Heart Association, accepted for publication.

Gonzalez-Rubio J, Zeevaert K, Buhl EM, Schedel M, Jockenhoevel S, Cornelissen CG, Wagner W, Thiebes AL. Transient iPSC reprogramming of bone marrow mesenchymal stromal cells reduces their neovascularization stimulation. Life Sciences, accepted for publication.

Schmidt M, Maié T, Cramer T, Costa IG, Wagner W. Cancer-associated fibroblasts reveal aberrant DNA methylation across different types of cancer. Clinical Epigenetics (2024); 16:164.

Plank A-C, Wiedmann M, Kuitunen-Paul S, Wagner W, Perez-Correa J-P, Franzen J, Charalampos I, Mirtschink P, Roessner V, Golub Y. DNA methylation and gene expression of immune cell markers in adolescents with chronic cannabis use: an exploratory study. BMC Psychiatry (2024) 24:676.

Tharmapalan V, Wagner W. Biomarkers for aging of blood - how transferable are they between mice and humans? Experimental Hematology (2024) 140:1046000.

Majewska J, Agrawal A, Mayo A, Roitman L, Chatterjee R, Kralova J, Landsberger T, Katzenelenbogen Y, Meir-Salame T, Hagai E, Sopher I, Perez-Correa JP, Wagner W, Maimon A, Amit I, Krizhanovsky UAV. p16-dependent increase of PD-L1 stability regulates immunosurveillance of senescent cells. Nature Cell Biology (2024) 26:1336-1345.

Hubens W, Kluge L, Seitz A, Verjans E, Rink L, Wagner W. Epigenetic Leukocyte Counts from Dried Blood Spots of Pediatric Patients. Clinical Chemistry (2024) 70:7.

Simm A, Dallmeier D, Wagner W. Biologisches Altern – die Hallmarks of Aging. In Geriatrie. Das gesamte Spektrum der Altersmedizin für Klinik und Praxis (2024) Bauer / Becker / Denkinger / Wirth. ISBN 978-3-17-041794-6, 2024

Elsafi Mabrouk M. H., Zeevaert K., Henneke A.-C., Maaßen C., Wagner W. Substrate elasticity does not impact on DNA methylation changes during differentiation of pluripotent stem cells. Cytotherapy. (2024).

Palikyras S, Sofiadis K, Stayropoulou A, Danieli-Mackay A, Varamogianni-Matasi V, Hörl D, Nasicionyte S, Zhu Y, Papadionysiou I, Papadakis A, Josipovic N, Zirkel A, O’Connell A, Loughran G, Keane J, Michel A, Wagner W, Beyer A, Harz H, Leonhardt H, Lukinavicious G, Nikolaou C, Papantonis A. Rapid and synchronous chemical induction of replicative-like senescence via a small molecule inhibitor. Aging Cell. (2024) 9:e14083.

Flosdorf N, Böhnke J, de Toledo MAS, Lutterbach N, Gómez Lerma V, Grasshoff M, Olschok K, Gupta S, Tharmapalan V, Schmitz S, Götz K, Schüler HM, Maurer A, Sontag S, Küstermann C, Sere K, Wagner W, Costa IG, Brümmendorf TH, Koschmieder S, Chatain N, Castilho M, Schneider RK, Zenke M. Proinflammatory phenotype of iPS cell-derived JAK2 V617F megakaryocytes induces fibrosis in 3D in vitro bone marrow niche. Stem Cell Reports. (2024) 19(2):224-238.

Kastberger B, Winter S, Brandstätter H, Biller J, Wagner W, Plesnila N. Treatment with Cerebrolysin Prolongs Lifespan in a Mouse Model of Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy. Adv Biol. (2024) 8(2):e2300439.

Hubens W H G, Maié T, Schnitker M, Bocova L, Puri D, Wessiepe M, Kramer J, Rink L, Koschmieder S, Costa I G, Wagner W. Targeted DNA methylation analysis facilitates leukocyte counts in dried blood samples. Clinical Chemistry. (2023) 69(11):1283-1294.

Jacobi H, Vieri M, Bütow M, Namasu CY, Flüter L, Costa IG, Maié T, Lindemann-Docter K, Chatain N, Beier F, Huber M, Wagner W, Crysandt M, Brümmendorf TH, Schemionek M. Myelofibrosis at diagnosis is associated with the failure of treatment-free remission in CML patients. Frontiers in Pharmacology. (2023) 14:1212392.

Bocova L, Hubens W, Engel C, Koschmieder S, Jost E, Wagner W. Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis. Clinical Epigenetics. (2023) 15:105.

Salz L, Seitz A, Schäfer D, Franzen J, Holzer T, Garcia-Prieto CA, Bürger I, Hardt O, Esteller M, Wagner W. Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome. Leukemia. (2023) 37(9):1868-1878. 

Gerardo-Nava JL, Jansen J, Günther D, Klasen L, Thiebes AL, Niessing B, Bergerbit C, Meyer AA, Linkhorst J, Barth M, Akhari P, Stingl J, Nagel S, Stiehl T, Lampert A, Leube R, Wessling M, Santoro F, Ingebrandt S, Jockenhövel S, Herrmann A, Fischer H, Wagner W, Schmidt RH, Kiessling F, Kramann R, De Laporte L. Transformative materials to create 3D functional human tissue models in vitro in a reproducible manner. Advanced Healthcare Materials. (2023) Jun 13:e2301030.

Desai P, Dasgupta A, Sofias AM, Peña Q, Göstl R, Slabu I, Schwaneberg U, Stiehl T, Wagner W, Jockenhövel S, Stingl J, Kramann R, Trautwein C, Brümmendorf TH, Kiessling F, Herrmann A, Lammers T. Transformative Materials for Interfacial Drug Delivery. Adv Healthc Mater. (2023) Jun 6:e2301062.

Puri D, Maaßen C, Varona Baranda M, Zeevaert K, Hahnfeld L, Hauser A, Fornero G, Elsafi Mabrouk MH, Wagner W. CTCF deletion alters the pluripotency and DNA methylation profile of human iPSCs. Frontiers in Cell and Developmental Biology-Stem Cell Research. (2023) 11:1302448.

Puri D, Wagner W. Epigenetic Rejuvenation by Partial Reprogramming, BioEssays. (2023) 45:e2200208.

Zeevaert K, Goetzke R, Elsafi Mabrouk MH, Schmidt M, Maaßen C, Henneke A-C, He C, Gillner A, Zenke M, Wagner W. YAP1 is 1 essential for self-organized differentiation of pluripotent stem cells. Biomaterials Advances. (2023) 146:213308.

Maié T, Schmidt M, Erz M, Wagner W, Costa IG. CimpleG: Finding simple CpG methylation signatures. Genome Biology. (2023) 24:161.

Schmidt M, Zeevaert K, Elsafi Mabrouk MH, Goetzke R, Wagner W. Epigenetic biomarkers to track differentiation of pluripotent stem cells. Stem Cell Reports. (2023) 18:145-158.

Vieri M, Tharmapalan V, Kalmer M, Baumeister J, Nicolic M, Schnitker M, Kirschner M, Flosdorf N, de Toledo M A S, Zenke M, Koschmieder S, Brümmendorf T H, Beier F, Wagner W. Cellular aging is accelerated in the malignant clone of myeloproliferative neoplasms. Blood Cancer Journal. (2023) 13(1):164.

Wiedmann M., Kuitunen-Paul S., Basedow L., Wolff M., DiDonato N., Franzen J., Wagner W., Roessner V., Golub Y. DNA methylation changes associated with cannabis use and verbal learning performance in adolescents: an exploratory whole genome methylation study. Translational Psychiatry. (2022) 12:317.

Perez-Correa J.-F., Tharmapalan V., Geiger H., Wagner W. Epigenetic clocks for mice based on age-associated regions that are conserved between mouse strains and human. Frontiers in Cell and Developmental Biology. (2022) 10:902857.

Ma Z., Szymansky De Toledo M., Wanek P., Elsafi Mabrouk M.H., Smet F., Pulak R., Pieske S., Piotrowski T., Herfs W., Brecher C., Schmidt R.H., Wagner W., Zenke M. Cell Cluster Sorting in Automated Differentiation of Patient-specific Induced Pluripotent Stem Cells Towards Blood Cells. Frontiers in Bioengineering and Biotechnology. (2022) 10:755983.

Neuberger E., Sontag S., Brahmer A., Philippi K.F.A., Radsak M.P., Wagner W., Simon P. Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy. Clinical Epigenetics. (2022) 14:29.

Elsafi Mabrouk M. H., Goetzke G., Abagnale G., Yesilyourt B., Salz L., Cypris O., Glück P., Liesenfelder S., Zeevaert K., Ma Z., Toledo M.A.S., Li R., Costa I.G., Lampert A., Pachauri V., Schnakenberg U., Zenke M., Wagner W. The spatial self-organization within pluripotent stem cell colonies is continued in detaching aggregates. Biomaterials. (2022) 282:121389.

Sontag S, Bocova L, Hubens WHG, Nüchtern S, Schnitker M, Look T, Schröder KM, Plümäkers B, Tharmapalan V, Wessiepe M, Kraus T, Kramer J, Rink L, Koschmieder S, Wagner W. Toward clinical application of leukocyte counts based on targeted DNA methylation analysis. Clinical Chemistry. (2022) 68:646-656. (Editorial featuring this study; Podcast on this study)

Božić T, Kuo C-C, Hapala J, Franzen J, Eipel M, Platzbecker U, Kirschner M, Beier F, Jost E, Thiede C, Wagner W. Investigation of Measurable Residual Disease in Acute Myeloid Leukemia by DNA Methylation Patterns. Leukemia. (2022) 36:80–89.

W. Wagner. How to translate DNA methylation biomarkers into clinical practice. W. Wagner, Frontiers in Cell and Developmental Biology. (2022) 10:854797.

Mayer F., Becker J., Reinauer C., Böhme P., Eickhoff S.B., Koop B., Gündüz T., Blum J., Wagner W., Ritz-Timme S. Altered DNA methylation at age‑associated CpG sites in children with growth disorders: impact on age estimation?International Journal of Legal Medicine. (2022) 136:987-996.

Cypris O., Franzen J., Frobel J., Glück P., Kuo C.-C., Schmitz S., Nüchtern S., Zenke M., Wagner W. Hematopoietic differentiation persists in human iPSCs defective in de novo DNA methylation. BMC Biology. (2022) 20:141.

Becker J, Böhme P, Reckert A, Eickhoff SB, Koop BE, Blum J, Gündüz T, Takayama M, Wagner W, Ritz-Timme S. Evidence for differences in DNA methylation between Germans and Japanese: Impact on epigenetic age estimation. International Journal of Legal Medicine. (2021) 136:405-413.

Franzen J, Georgomanolis T, Selich A, Kuo CC, Stöger R, Brand L, Mulabdic MS, Fernandez-Rebollo E, Grezella C, Ostrowska A, Begemann M, Nicolic M, Rath B, Ho AD, Rothe M, Schambach A, Papantonis A, Wagner W. DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift. Communications Biology. (2021) 4: 598.

Franzen J, Nüchtern S, Tharmapalan V, Vieri M, Nikolic M, Han Y, Balfanz P, Marx N, Dreher M, Brümmendorf TH, Dahl E, Beier F, Wagner W. Epigenetic clocks are not accelerated in COVID-19 patients. International Journal of Molecular Sciences. (2021) 22: 9306.

Naue J, Pfeifer M, Augustin C, Becker J, Fleckhaus J, Grabmüller M, Han Y, Heidorn F, Hollaender O, Klein-Unseld R, Kulstein G, Lichtenwald J, Neubauer J, Suarez P, Haas C, Schneider PM, Vennemann M, Böhme P, Arbeitsgemeinschaft Molekulare Altersschätzung der Deutschen Gesellschaft für Rechtsmedizin (DGRM). Forensische DNA-Methylierungsanalyse: Zweiter, technischer Ringversuch der Arbeitsgruppe „Molekulare Altersschätzung“ der Deutschen Gesellschaft für Rechtsmedizin, Rechtsmedizin. (2021) 31: 202–216. 

Toledo MAS, Gatz M, Sontag S, Gleixner KV, Eisenwort G, Feldbarg k, Hamouda AEI, Kluge F, Guareschi R, Rossetti G, Sechi AS, Dufva OMJ, Mustjoki SM, Maurer A, Schüler HM, Goetzke R, Braunschweig T, Simonowski A, Panse J, Jawhar M, Reiter A, Hilberg F, Ettmayer P, Wagner W, Koschmieder S, Brümmendorf TH, Valent P, Chatain N, Zenke M. Nintedanib Targets KIT D816V Neoplastic Cells Derived from Induced Pluripotent Stem cells of Systemic Mastocytosis. Blood. (2021) 137: 2070–2084.

Schmidt M, Maixner F, Hotz G, Pap I, Szikossy I, Palfi G, Zink A, Wagner W. DNA Methylation Profiling in Mummified Human Remains from the Eighteenth-Century. Scientific Reports. (2021) 11: 15493.

Sibert NT, Ferreira MSV, Wagner W, Eipel M, Brümmendorf TH, Orlikowsky T, Beier F. Telomere length and epigenetic age of preterm neonates as potential biomarkers for the-/indicators of- organismal growth in preterm neonates. Experimental and Therapeutic Medicine. (2021) 21: 344.

Siahaan T, Reckert A, Becker J, Eickhoff SB, Koop B, Gündüz T, Böhme P, Mayer F, Küppers L, Wagner W, Ritz-Timme S. Molecular and morphological findings in a sample of oral surgery patients: What can we learn for multivariate concepts for age estimation?Journal of Forensic Sciences. (2021) 66: 1524-1532.

Han Y, Nikolić M, Gobs M, Franzen J, de Haan G, Geiger H, Wagner W. Targeted methods for epigenetic age-predictions in mice. Scientific Reports. (2020) 10: 22439.

Schmidt M, Maié T, Dahl E, Costa IG, Wagner W. Deconvolution of cellular subsets in human tissue based on targeted DNA methylation analysis at individual CpG sites. BMC Biology. (2020) 18: 178.

Cypris O, Eipel O, Franzen J, Rösseler C, Tharmapalan V, Kuo CC, Vieri M, Nikolić M, Kirschner M, Brümmendorf TH, Zenke M, Lampert A, Beier F, Wagner W. PRDM8 reveals aberrant DNA methylation in aging syndromes and is relevant for hematopoietic and neuronal differentiation. Clinical Epigenetics. (2020) 12: 125.

Florian MC, Leins H, Gobs M, Han Y, Marka G, Soller K, Vollmer A, Sakk V, Nattamai KH, Rayes A, Zhao X, Setchell K, Mulaw M, Wagner W, Zheng Y, Geiger H. Inhibition of Cdc42 activity extends lifespan and decreases circulating inflammatory cytokines in aged female C57BL/6 mice. Aging Cell. (2020) 9: e13208.

Fernandez-Rebollo E, Franzen J, Goetzke R, Hollmann J, Ostrowska A, Oliverio M, Sieben T, Rath B, Kornfeld J-W, Wagner W. Senescence-associated metabolomic phenotype in primary and iPSC-derived mesenchymal stromal cells. Stem Cell Reports. (2020) 2: 201-209.

Han Y, Franzen J, Stiehl T, Gobs M, Kuo CC, Nikolic M, Hapala J, Koop BE, Strathmann K, Ritz-Timme S, Wagner W. New Targeted Approaches for Epigenetic Age Predictions. BMC Biology. (2020) 18: 71.

Hollmann J, Brecht J, Goetzke R, Franzen J, Selich A, Schmidt M, Eipel M, Ostrowska A, Hapala J, Fernandez-Rebollo E, Müller-Newen G, Rothe M, Eggermann T, Zenke M, Wagner W. Genetic Barcoding Reveals Clonal Dominance in iPSC-Derived Mesenchymal Stromal Cells. Stem Cell Research & Therapy. (2020) 11: 105: 

Kloubert V, Wessels I, Wolf J, Blaabjerg K, Janssens V, Hapala J, Wagner W, Rink L. Zinc deficiency leads to reduced interleukin-2 production by active gene silencing due to enhanced CREMα expression in T cells. Clinical Nutrition. (2020) S0261-5614(20)30598-7.

Koop BE, Mayer F, Gündüz T, Blum J, Becker J, Schaffrath J, Wagner W, Han Y, Boehme P, Ritz-Timme S. Postmortem age estimation via DNA methylation analysis in buccal swabs from corpses in different stages of decomposition - A “proof of principle” study. International Journal of Legal Medicine. (2020) epub ahead of print. 

Koop BE, Reckert A, Becker J, Han Y, Wagner W, Ritz-Timme S. Epigenetic clocks may come out of rhythm – Implications for the estimation of chronological age in forensic casework. International Journal of Legal Medicine. (2020) 34(6): 2215-2228.

Zeevaert K, Elsafi Mabrouk MH, Wagner W, Goetzke R. Cell mechanics in embryoid bodies. Cells. (2020) 9(10): 2270.

Bell CG, Lowe R, Adams PD, Baccarelli AA, Beck S, Bell JT, Christensen BC, Gladyshev VN, Heijmans BT, Horvath S, Idecker T, Issa P-J, Kelsey KT, Marioni RE, Reik W, Relton CL, Schalkwyk LC, Teschendorff AE, Wagner W, Zhang K, Rakyan VK. DNA methylation aging clocks: challenges and recommendations.Genome Biology. (2019) 20: 249

Goetzke R, Keijdener H, Franzen J, Ostrowska A, Nüchtern S, Mela P, Wagner W. Differentiation of Induced Pluripotent Stem Cells towards Mesenchymal Stromal Cells is Hampered by Culture in 3D Hydrogels. Scientific Reports. (2019) 9:15578.

Cypris O., Božić T., Wagner W. Chicken or Egg: Is Clonal Hematopoiesis Primarily Caused by Genetic or Epigenetic Aberrations? Front Genet. (2019) 10:785.

Brown A, Schuetz D, Han Y, Daria D, Nattamai KJ, Eiwen K, Sakk V, Pospiech J, Saller T, van Zant G, Wagner W, Geiger H. The lifespan quantitative trait locus gene securin controls hematopoietic progenitor cell function. Haematologica. (2019) Vol. 105 No.2.

Meents JE, Bressan E, Sontag S, Foerster A, Hautvast P, Rösseler C, Hampl M, Schüler H, Goetzke R, Le TKC, Kleggeveit IP, Cann KL, Kerth C, Rush AM, Gogers M, Kohl Z, Schmelz M, Wagner W, Jørum E, Mamer B, Winner B, Zenke M, Lampert A. The role of Nav1.7 in human nociceptors: insights from human iPS cell-derived sensory neurons of erythromelalgia patients. Pain. (2019) 160:1327-1341. 

Eipel M, Božić T, Mies A, Beier F, Jost E, Brümmendorf TH, Platzbecker U, Wagner W. Tracking of myeloid malignancies by targeted analysis of successive DNA methylation at neighboring CG dinucleotides. Haematologica. (2019) 104:e349-e351.

Cypris O, Frobel J, Rai S, Franzen J, Sontag S, Goetzke R, Symanski de Toledo MA, Zenke M, Wagner W. Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells. Clinical Epigenetics. (2019) 11:19. 

Wagner W. The Link between Epigenetic Clocks for Aging and Senescence. Frontiers in Genetics. (2019) 3:303.

Božić T, Frobel J, Raic A, Ticconi F, Kuo CC, Heimann-Heimbach S, Goecke TW, Zenke M, Jost E, Costa IG, Wagner W. Variants of DNMT3A cause transcript-specific DNA methylation patterns and affect hematopoiesis. Life Science Alliance. (2018) 1(6):e201800153.

Marioni R, Belsky DW, Deary IJ, Wagner W. Association of Facial Aging with DNA Methylation and Epigenetic Age Predictions. Clinical Epigenetics. (2018) 10: 140.

Sheng C, Jungverdorben J, Wiethoff H, Lin Q, Flitsch L, Eckert D, Hebisch M, Fischer J, Kevasan J, Weykopf B, Schneider L, Holtkamp D, Beck H, Till A, Wuellner U, Ziller M, Wagner W, Peitz M, Brüstle O. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation. Nature Communications. (2018) 9: 4047.

Han Y, Eipel M, Franzen J, Sakk V, Dethmers-Ausema B, Yndriago L, Izeta A, de Haan G, Geiger H, Wagner W. Epigenetic age-predictor for mice based on three CpG sites. Elife. (2018) 7. pii: e37462. 

Jakob F, Ebert R, Fernandez-Rebollo E, Bischof O, Wagner W. Response to Letter to the Editor of JBMR: Epigenetic Aging in Osteoporosis. Journal of Bone and Mineral Research. (2018) 33: 1904-1905.

Göbel C, Goetzke R, Eggermann T, Wagner W. Interrupted reprogramming into induced pluripotent stem cells does not rejuvenate human mesenchymal stromal cells. Scientific Reports. (2018) 8: 11676.

Frobel J, Rahmig S, Franzen J, Waskow C, Wagner W. Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice. Clinical Epigenetics. (2018) 10: 67.

Oberacker T, Bajorat J, Ziola S, Schroeder A, Röth D, Kastl L, Edgar BA, Wagner W, Gülow K, Krammer PH. Enhanced expression of thioredoxin-interacting-protein regulates oxidative DNA damage and aging. FEBS Lett. (2018) 592: 2297-2307.

Lubberich RK, Walenda T, Goecke TW, Strathmann K, Isfort S, Brümmendorf TH, Koschmieder S, Wagner W. Serum of myeloproliferative neoplasms stimulates hematopoietic stem and progenitor cells. PLoS ONE. (2018) 13: e0197233.

Grezella C, Fernandez-Rebollo E, Franzen J, Ventura Ferreira MS, Beier F, Wagner W. Effects of Senolytic Drugs on Human Mesenchymal Stromal Cells. Stem Cell Research & Therapy. (2018) 9: 108.

Goetzke R, Sechi A, de Laporte L, Neuss-Stein S, Wagner W. Why the impact of mechanical stimuli on stem cells remains a challenge. Cellular and Molecular Life Sciences. (2018) 75: 3297-3312.

Schmidt E, Dhaouadi I, Gaziano I, Oliverio M, Klemm P, Awazawa M, Mitterer G, Fernandez-Rebollo E, Pardas-Juni M, Wagner W, Hammerschmidt P, Loureiro R, Kiefer CA, Hansmeier NR, Khani S, Bergami B, Heine M, Ntini E, Frommolt P, Zentis P, Ørom U, Heeren J, Blüher M, Bilban M, Kornfeld JW. LincRNA H19 protects from dietary obesity by constraining expression of monoallelic genes in brown fat. Nature Communications. (2018) 9: 3622.

Zirkel A, Nikolic M, Sofiadis K, Mallm J-P, Brackley CA, Gothe H, Drechsel O, Becker C, Altmüller J, Josipovic N, Georgomanolis T, Brant L, Franzen J, Koker M, Gusmao EG, Costa IG, Ullrich RT, Wagner W, Roulos V, Nürnberg P, Marenduzzo D, Rippe K, Papantonis A. HMGB2 loss upon senescence entry disrupts genomic organization and induces CTCF clustering across cell types. Molecular Cell. (2018) 70: 1-15.

Li J, Garcia CC, Riedt T, Brandes M, Szczepanski S, Bossart P, Wagner W, Janzen V. Murine hematopoietic stem cell reconstitution potential is maintained by osteopontin during aging. Scientific Reports. (2018) 8: 2833.

Goetzke R, Franzen J, Ostrowska A, Vogt M, Blaeser A, Klein G, Rath B, Fischer H, Zenke M, Wagner W. Does Soft Really Matter? Differentiation of Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells is Not Influenced by Soft Hydrogels. Biomaterials. (2018) 156: 147-158.

Frobel J, Bozic T, Lenz M, Uciechowski P, Han Y, Herwartz R, Strathmann K, Isfort S, Panse J, Esser A, Birkhofer C, Gerstenmaier U, Kraus T, Rink L, Koschmieder S, Wagner W. Leukocyte counts based on DNA methylation at individual cytosines. Clinical Chemistry. (2018) 10: 67.

Fernandez-Rebollo E, Eipel M, Seefried L, Hoffmann P, Strathmann, K, Jakob F*, Wagner W*. Primary osteoporosis is not reflected by disease-specific DNA. Journal of Bone and Mineral Research. (2018) 33:356-361.

Synthetische Biologie, Standortbestimmung. Positionspapier der Ständigen Senatskommission für Grundsatzfragen der Genforschung, 2018

Translation – von der Stammzelle zur innovativen Therapie. Analyse und Empfehlungen zur klinischen Translation von Stammzellforschung in Deutschland. White Paper des German Stem Cell Networks, Arbeitsgruppe Translation stammzellbasierter Therapien, 2018

Abagnale G, Sechi A, Steger M, Zhou Y, Kuo CC, Aydin G, Schalla C, Müller-Newen G, Zenke M, Costa IG, van Rijn P, Gillner A, Wagner W. Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies. Stem Cell Reports. (2017) 9:654-666.

Ventura Ferreira MS, Crysandt M, Ziegler P, Hummel S, Wilop S, Kirschner M, Schemionek M, Jost E, Wagner W, Brümmendorf TH, Beier F. Evidence for a pre-existing telomere deficit in non-clonal hematopoietic stem cells in patients with acute myeloid leukemia. Annals of Hematology. (2017) 96: 1457-1461.

Fernandez-Rebollo E, Mentrup B, Ebert R, Franzen J, Abagnale G, Sieben T, Hoffmann P, Roux PF, Rath B, Goodhardt M, Lemaitre J-M, Bischof O, Franz J, Wagner W. Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells. Scientific Reports. (2017) 7: 5132.

Franzen J, Zirkel A, Blake J, Rath B, Benes V, Papantonis A, Wagner W. Senescence-Associated DNA Methylation is Stochastically Acquired in Subpopulations of Mesenchymal Stem Cells. Aging Cell. (2017) 16: 183-191.

Rose M, Kloten V, Noetzel E, Gola L, Ehling J, Heide T, Meurer  SK, Gaiko-Shcherbak A, Sechi AS, Huth S, Weiskirchen R, Klaas O, Antonopoulos W, Lin Q, Wagner W, Veeck J, Gremse F, Steitz J, Knüchel R, Dahl E. ITIH5 mediates epigenetic reprogramming of breast cancer cells. Molecular Cancer. (2017) 16:44.

Zhang Y, Hapala J, Brenner H, Wagner W. Individual CpG Sites that are Associated with Age and Life Expectancy become Hypomethylated upon Aging. Clinical Epigenetics. (2017) 9: 9.

Wagner W. Epigenetic aging clocks in mice and men. Genome Biology. (2017) 18: 107.

Moskalev A, Anisimov V, Aliper A, Artemov A, Asadullah K, Belsky D, Baranova A, de Grey A, Dixit VD, Debonneuil E, Dobrovolskaya E, Fedichev P, Fedintsev A, Fraifeld V, Franceschi C, Freer R, Fülöp T, Feige J, Gems D, Gladyshev V, Gorbunova V, Irincheeva I, Jager S, Jazwinski SM, Kaeberlein M, Kennedy B, Khaltourina D, Kovalchuk I, Kovalchuk O, Kozin S, Kulminski A, Lashmanova E, Lezhnina K, Liu GH, Longo V, Mamoshina P, Maslov A, Pedro de Magalhaes J, Mitchell J, Mitnitski A, Nikolsky Y, Ozerov I, Pasyukova E, Peregudova D, Popov V, Proshkina E, Putin E, Rogaev E, Rogina B, Schastnaya J, Seluanov A, Shaposhnikov M, Simm A, Skulachev V, Skulachev M, Solovev I, Spindler S, Stefanova N, Suh Y, Swick A, Tower J, Gudkov AV, Vijg J, Voronkov A, West M, Wagner W, Yashin A, Zemskaya N, Zhumadilov Z, Zhavoronkov A. A review of the biomedical innovations for healthy longevity. Aging (Albany NY). (2017) 9: 7-25.

Kalwa M, Hänzelmann S, Otto S, Kuo Chao-Chung, Franzen J, Joussen S, Fernandez Rebollo E, Rath B, Koch C, Hofmann A, Lee S-H, Teschendorff A, Denecke B, Lin Q, Widschwendter M, Weinhold E, Costa I, and Wagner W. The lncRNA HOTAIR Impacts on Mesenchymal Stem Cells via Triple Helix Formation.Nucleic Acid Research. (2016) 44: 10631-10643.

Wilop S, Chou W-C, Jost E, Crysandt M, Panse J, Chuang M-K, Brümmendorf T H, Wagner W, Tien H-F, Kharabi Masouleh B. A three-gene expression based risk score can refine the European LeukemiaNet AML classification.Journal of Hematology & Oncology. (2016) 9: 78.

Eipel M, Mayer F, Arent T, Ferreira MRP, Birkhofer C, Gerstenmaier U, Costa IG, Ritz-Timme S, Wagner W. Epigenetic age predictions based on buccal swabs are more precise in combination with cell type-specific DNA methylation signatures. Aging US. (2016) 8: 1034-1048.

Wagner W, Frobel J, Goetzke R. Epigenetic Quality Check – How Good are Your Mesenchymal Stromal Cells?Epigenomics. (2016) 8: 889-894.

Vasko T, Frobel J, Lubberich R, Goecke TW, Wagner W. iPSC-Derived Mesenchymal Stromal Cells are Less Supportive than Primary MSCs for Co-Culture of Hematopoietic Progenitor Cells. Journal of Hematology & Oncology. (2016) 9: 43.

Franzen J, Wagner W, Fernandez-Rebollo E. Epigenetic Modifications upon Senescence of Mesenchymal Stem Cells. Current Stem Cell Reports. (2016) 2: 248–254.

Lin Q, Weidner CI, Costa I, Marioni R, Ferreira M, Deary I and Wagner W. DNA methylation levels at individual age-associated CpG sites can be indicative for life expectancy. Aging. (2016) 8: 394-401.

Weidner CI, Lin Q, Birkhofer C, Gerstenmaier U, Kaifie A, Kirschner M, Bruns H, Balabanov S, Trummer A, Stockklausner C, Höchsmann B, Schrezenmeier H, Wlodarski M, Panse J, Brümmendorf TH, Beier F, Wagner W. DNA methylation in PRDM8 is indicative for dyskeratosis congenita. Oncotarget. (2016)7: 10765-10772.

Candio de Almeida D, Ferreira MRP, Franzen J, Weidner C, Frobel J, Zenke M, Costa IG, Wagner W. Epigenetic classification of human mesenchymal stromal cells. Stem Cell Reports. (2016) 6: 168-75.

Wagner W, Fernandez-Rebollo E, Frobel J. DNA-Methylation Changes in Replicative Senescence and Aging: Two Sides of the Same Coin?Epigenomics. (2016) 8: 1-3.

Schulze I, Rohde C, Scheller M, Krause A, Herbst F, Riemke P, Hebestreit K, Tschanter P, Lin Q, Linhart H, Godley L, Glimm H, Dugas M, Wagner W, Bredel W, Rosenbauer R, Müller-Tidow C. Increased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis.Blood. (2016) 127: 1575-86.

Božić T, Lin Q, Frobel J, Wilop S, Hoffmann M, Müller-Tidow C, Brümmendorf T H, Jost E, Wagner W. DNA-Methylation in C1R is a Prognostic Biomarker for Acute Myeloid Leukemia. Clinical Epigenetics. (2015) 7: 116.

Teschendorff AE, Jones A, Lee SH, Fiegl H, Kalwa M, Wagner W, Chindera K, Evans I, Dubeau L, Orjalo A, Horlings HM, Niederreiter L, Kaser A, Yang W, Goode EL, Fridley BL, Jenner RG, Berns EMJJ, Wik E, Salvesen HB, Wisman GBA, van der Zee AGJ, Davidson B, Trope CG, Lambrechts S, Vergote I, Calvert H, Jacobs IJ, Widschwendter M. HOTAIR and its Surrogate DNA Methylation Signature indicate Carboplatin Resistance in Ovarian Cancer. Genome Medicine. (2015) 7: 108.

Diener Y, Walenda T, Jost E, Brümmendorf TH, Bosio A, Wagner W, Bissels U. MicroRNA expression profiles of serum from patients before and after chemotherapy. Genomics Data. (2015) 6: 125-127.

Erdmann G, Suchanek M, Horn P, Graf F, Volz C, Horn T, Zhang X, Wagner W, Ho AD, Boutros M. Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi Screening. Genome Medicine. (2015) 7:46 

Lin Q, Wagner W. Epigenetic Aging Signatures are Coherently Modified in Cancer.  PLoS Genetics. (2015) 11: e1005334.

Abagnale G, Steger M, Nguyen VH, Hersch N, Sechi A, Joussen S, Denecke B, Merkel R, Hoffmann B, Dreser A, Schnakenberg U, Gillner A, Wagner W. Surface Topography Enhances Differentiation of Mesenchymal Stem Cells Towards Osteogenic and Adipogenic Lineages. Biomaterials. (2015) 61: 316-26.

Hänzelmann S, Beier F, Gusmao EG, Koch CM, Hummel S, Charapitsa I, Joussen S, Benes B, Brümmendorf TH, Reid G, Costa IG, Wagner W. Replicative Senescence is Associated with Nuclear Reorganization and with DNA Methylation at Specific Transcription Factor Binding Sites. Clinical Epigenetics. (2015) 7: 19.

Lenz M, Goetzke R, Schenk A, Schubert C, Veeck J, Hemeda H, Koschmieder S, Zenke M, Schuppert A, Wagner W. Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells. Scientific Reports. (2015) 5: 8973. 

Steger M, Abagnale G, Bremus-Köbberling E, Wagner W, Gillner A. Nanoscale biofunctionalization of polymer surfaces by laser treatment for controlled cellular differentiation; in König/Ostendorf (Eds.), Optically Induced Nanostructures. Biomedical and Technical Applications, De Gruyter. (2015) ISBN 978-3-11-038350-8, Berlin; 2015.

Walenda T, Diener Y, Jost E, Morin-Kensicki E, Goecke TW, Bosio A, Rath B, Brümmendorf TH, Bissels U, Wagner W. MicroRNAs and Metabolites in Serum Change after Chemotherapy: Impact on Hematopoietic Stem and Progenitor Cells. PLoS ONE. (2015) 10: e0128231.

Weidner CI, Ziegler P, Hahn M, Brümmendorf TH, Ho AD, Dreger P, Wagner W. Epigenetic aging upon allogeneic transplantation: The hematopoietic niche does not affect age-associated DNA methylation. Leukemia. (2015) 29: 985-8.

Reinisch A, Etchart N, Hofmann NA, Thomas D, Fruehwirth M, Sinha S, Hartwig UF, Beham-Schmid C, Trajanoski S, Lin Q, Wagner W, Dullin C, Alves F, Schallmoser K, Andreeff M, Strunk M. Epigenetic and in vivo comparison of diverse mesenchymal stromal cell sources reveals an endochondral signature for human hematopoietic niche formation. Blood. (2015) 125: 249-60.

Wagner W, Weidner CI, Lin Q. Do age-associated DNA methylation changes increase the risk of malignant transformation? BioEssays. (2015) 37:20-4.

Wuchter P, Bieback K, Schrezenmeier H, Bornhäuser M, Müller LP, Bönig H, Wagner W, Meisel R, Paveli P, Tonn T, Lang P, Müller I, Renner M, Malchereka G, Saffrich R, Buss E, Horn P, Rojewskic M, Schmitt A, Ho AD, Sanzenbacher R, Schmitt M. Standardization of GMP-compliant production of bone marrow derived human mesenchymal stromal cells. Cytotherapy. (2015) 17(2):128-39.

Frobel J, Hemeda H, Lenz M, Abagnale G, Joussen S, Denecke B, Šarić T, Zenke M, Wagner W. Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells. Stem Cell Reports. (2014) 3: 414-422. 

Qin J, Sontag S, Lin Q, Mitzka S, Leisten I, Schneider RK, Wang X, Jauch A, Peitz M, Brüstle O, Wagner W, Zhao RC, Zenke M. Cell Fusion Enhances Mesendodermal Differentiation of Human Induced Pluripotent Stem Cells. Stem Cells Dev. (2014) 23(23):2875-82.

Weidner CI, Wagner W. The Epigenetic Tracks of Aging. Biol. Chem. (2014) 395: 1307-1314.

Abnaof K, Mallela N, Walenda G, Meurer S, Seré K, Lin Q, Smeets B, Hoffmann K, Wagner W, Zenke M, Weiskirchen R, Fröhlich H. TGF-beta Stimulation in Human and Murine Cells Reveals Commonly Affected Biological Processes and Pathways at Transcription Level. BMC Systems Biology. (2014) 8: 55.

Schellenberg A, Joussen S, Moser K, Hampe N, Hersch N, Hemeda H, Schnitker J, Denecke B, Lin Q, Pallua N, Zenke M, Merkel R, Hoffmann B, Wagner W. Matrix Elasticity, Replicative Senescence and DNA Methylation Patterns of Mesenchymal Stem Cells. Biomaterials. (2014) 35: 6351.

Schellenberg A, Mauen S, Koch CM, Jans R, de Waele P, Wagner W. Proof of Principle: Quality Control of Therapeutic Cell Preparations using Senescence-Associated DNA-Methylation Changes. BMCResearch Notes. (2014) 23: 25.

Walenda T*, Stiehl T*, Braun H, Fröbel J, Ho AD, Schroeder T, Goecke TW, Rath B, Germing U, Marciniak-Czochra A, Wagner W. Feedback Signals in Myelodysplastic Syndromes: Increased Self-Renewal of the Malignant Clone Suppresses Normal Hematopoiesis. PLoS Computational Biology. (2014) 10: e1003599.

Weidner CI, Lin Q, Koch CM, Eisele L, Beier F, Ziegler P, Bauerschlag DO, Jöckel KH, Erbel R, Mühleisen TW, Zenke M, Brümmendorf TH, Wagner W. Aging of blood can be tracked by DNA methylation changes at just three CpG sites. Genome Biology. (2014) 15: R24.

Radtke S, Giebel B, Wagner W, Horn P. Platelet Lysates and their Role in Cell Therapy. ISBT Science Series. (2014)

Schellenberg A, Ross R, Abagnale G, Joussen S, Schuster P, Arshi A, Pallua N, Jockenhoevel, S, Gries T, Wagner W. 3D Non-Woven Polyvinylidene Fluoride Scaffolds: Fibre Cross Section and Texturizing Patterns have Impact on Growth of Mesenchymal Stromal Cells. PLoS ONE. (2014) 9: e94353

Jost E, Lin Q, Weidner CI, Wilop S, Hoffmann M, Walenda T, Schemionek M, Herrmann O, Zenke M, Brümmendorf T, Koschmieder S., Wagner W. Epimutations Mimic Genomic Mutations of DNMT3A in Acute Myeloid Leukemia. Leukemia (2014) 28: 1227-1234.

Ludwig A, Saffrich R, Eckstein V, Bruckner T, Wagner W, Ho AD, Wuchter P. Functional potentials of human hematopoietic progenitor cells are maintained by mesenchymal stromal cells and not impaired by Plerixafor. Cytotherapy. (2014) 16(1):111-21.

Hemeda H, Giebel B, Wagner W. Evaluation of Human Platelet Lysate versus Fetal Bovine Serum for Culture of Mesenchymal Stromal Cells. Cytotherapy. (2014) 16(2):170-80.

Drescher, W., Knobe, M., Wagner, W. & Pufe, T. (2014). New therapies of bone necrosis. In Springer eBooks. eISBN 978-3-642-357671.S. 273–275. https://doi.org/10.1007/978-3-642-35767-1_37.

Drescher, W., Knobe, M., Wagner, W. & Pufe, T. (2014). Animal Models of Bone Necrosis: An Overview. In Springer eBooks. eISBN 978-3-642-357671. S. 507–510. https://doi.org/10.1007/978-3-642-35767-1_65.

Weidner CI, Walenda T, Lin Q, Wölfler MM, Denecke B, Gesteira Costa I, Zenke M, Wagner W. Hematopoietic Stem and Progenitor Cells Acquire Distinct DNA-Hypermethylation During in vitro Culture. Scientific Reports. (2013) 3:3372.

Fuellen G, Boerries M, Busch H, de Grey AD, Hahn U, Hiller T, Hoeflich A, Jansen L, Janssens GE, Kaleta C, Meinema AC, Schäuble S, Schofield PN, Smith B, Sühnel J, Vera J, Wagner W, Wönne EC, Wuttke D, Simm A. In-Silico-Approaches and the Role of Ontologies in Aging Research. Rejuvenation Research. (2013) 16(6):540-6.

Walenda G, Abnaof K, Joussen S, Meurer S, Smeets H, Rath B, Hoffmann K, Fröhlich H, Zenke M, Weiskirchen R, Wagner W. TGF-beta1 does not induce senescence of multipotent mesenchymal stromal cells and has similar effects in early and late passages. PLoS One. (2013) 8(10):e77656.

Hemeda H*, Kalz J*, Walenda G*, Lohmann M, Wagner W. Heparin concentration is critical for cell culture with human platelet lysate. Cytotherapy. (2013) 15(9):1174-81.

Willmann CA, Hemeda H, Pieper LA, Lenz M, Qin J, Joussen S, Sontag S, Wanek P, Denecke B, Schüler HM, Zenke M, Wagner W. To clone or not to clone? Induced pluripotent stem cells can be generated in bulk culture. PLoS ONE. (2013) 8(5): e65324.

Koch CM, Wagner W. Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells. Methods in Molecular Biology. (2013) 1048: 309-321.

Lin Q, Wagner W, Zenke M. Analysis of human genome-wide DNA methylation profiles by BeadChip technology. Methods in Molecular Biology. (2013) 23(2): 248-259.

Koch CM, Reck K, Shao K, Lin Q, Joussen S, Ziegler P, Walenda G, Drescher W, Opalka B, May T, Brümmendorf T, Zenke M, Saric T, Wagner W. Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes. Genome Research. (2013) 23(2): 248-259.

Shao K, Koch CM, Gupta MK, Lin Q, Lenz L, Laufs S, Denecke B, Schmidt M, Linke M, Hennies HC, Hescheler J, Zenke M, Zechner U, Šarić T*, Wagner W* (2012). Induced Pluripotent Mesenchymal Stromal Cell Clones Retain Donor-Derived Differences in DNA Methylation Profiles. Molecular Therapy. (2013) 21(1): 240-250.

Ventura Ferreira MS, Schneider RK, Wagner W, Jahnen-Dechent W, Labude N, Bovi M, Piroth DM, Knüchel R, Hieronymus T, Müller AM, Zenke M, Neuss S. 2D Polymer-based Cultures expand Cord Blood-derived Hematopoietic Stem Cells and Support Engraftment of NSG mice. Tissue Eng Part C Methods. (2013) 19(1):25-38.

Schellenberg A, Hemeda H, Wagner W. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency. Methods in Molecular Biology. (2013) 976: 143-154.

Ventura-Fereira M, Labude N, Walenda G, Adamzyk C, Wagner W, Pieroth D, Müller AM, Knüchel R, Hieronymus T, Zenke M, Jahnen-Dechent W, Neuss S. Ex vivo Expansion of Cord Blood-CD34+ Cells Using IGFBP2 and Angptl-5 Impairs Short-term Lymphoid Repopulation In vivo. J Tissue Eng and Reg Med. J Tissue Eng Regen Med (2013) 7(12):944-54.

Kramer J, Dazzi F, Dominici M, Schlenke P, Wagner W. Clinical Perspectives of Mesenchymal Stem Cells. Stem Cells International. (2012) Article ID 684827.

Wagner W. Implications of long-term culture for mesenchymal stem cells: genetic defects or epigenetic regulation?Stem Cell Research & Therapy. (2012) 3(6): 54.

Walenda G*, Hemeda H*, Schneider RK, Merkel R, Hoffmann B, Wagner W. Human Platelet Lysate Gel Provides a Novel 3D-Matrix for Enhanced Culture Expansion of Mesenchymal Stromal Cells. Tissue Engineering. (2012) 18(12): 924-934.

Zepeda-Moreno A, Saffrich R, Walenda T, Hoang VT, Wuchter P, Sánchez-Enríquez S, Corona-Rivera A, Wagner W and Ho AD. Modeling SDF-1 induced mobilization in leukemia cell lines. Experimental Hematology. (2012) 40(8): 666-674.

Lohmann M, Walenda G, Hemeda H, Joussen S, Drescher W, Jockenhoevel S, Hutschenreuter G, Zenke M and Wagner W. Donor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells. PLoS ONE. (2012) 7(5): e37839.

Koch CM, Joussen S, Schellenberg A, Lin Q, Zenke M, Wagner W. Monitoring of cellular senescence by DNA-methylation at specific CpG sites. Aging Cell. (2012) 11(2): 366-369.

Bissels U, Bosio A, Wagner W. MicroRNAs are shaping the hematopoietic landscape. Haematologica. (2012) 97(2): 160-167.

Leisten I, Kramann R, Ventura Ferreira MS, Bovi M, Neuss S, Ziegler P, Wagner W, Knüchel R, Schneider RK. Three-dimensional co-culture of hematopoietic stem and progenitor cells and mesenchymal stem cells in collagen scaffolds: An in vitro model of the hematopoetic niche. Biomaterials. (2012) 33(6): 1736-1747.

Schellenberg A, Stiehl T, Horn P, Joussen S, Pallua N, Ho AD, Wagner W. Population dynamics of mesenchymal stromal cells during culture expansion. Cytotherapy. (2012) 14(4): 401-411.

Koch C and Wagner W. Epigenetic-Aging-Signature to determine age in different tissues. Aging. (2011) 3: 1-10. 

Schellenberg A, Lin Q, Schueler H, Koch CM, Joussen S, Denecke B, Walenda G, Pallua N, Suschek CV, Zenke M, and Wagner W. Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks. Aging. (2011) 3(9): 873-888.

Diehlmann A, Bork S, Saffrich R, Veh RW, Wagner W, Derst C. K-ATP channels in mesenchymal stromal stem cells: strong up-regulation of Kir6.2 subunits upon osteogenic differentiation. Tissue and Cell. (2011) 43(5): 331-336.

Wirz S, Dietrich M, Flanagan TC, Bokermann G, Wagner W, Schmitz-Rode T, Jockenhoevel S. Influence of PDGF-AB on Tissue Development in Autologous Platelet-Rich Plasma Gels. Tissue Engineering. (2011) 17(13-14): 1891-1899.

Bauerschlag DO, Ammerpohl O, Bräutigam K, Schem C, Li Q, Weigel MT, Hilpert F, Arnold N, Maass N, Meinhold-Heerlein I, Wagner W. Progression-Free Survival in Ovarian Cancer is Reflected in Epigenetic DNA-Methylation Profiles. Oncology. (2011) 80(1-2): 12-20.

Walenda T, Bokermann G, Jost E, Galm O, Schellenberg A, Koch CM, Piroth DM, Drescher W, Brümmendorf TH, Wagner W. Serum after autologous transplantation stimulates proliferation and expansion of human hematopoietic progenitor cells. PLoS ONE. (2011) 6(3): e18012.

Walenda T*, Bokermann G*, Ventura Ferreira MS, Piroth DM, Hieronymus T, Neuss S, Zenke M, Ho AD, Müller AM, Wagner W. Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem and progenitor cells. Experimental Hematology. (2011) 39(6): 617-628.

Koch C*, Suschek CV*, Bork S, Lin Q, Görgens M, Joussen S, Pallua N, Ho AD, Zenke M, Wagner W. Specific age-associated DNA methylation changes in human dermal fibroblasts. PLoS ONE. (2011) 6(2): e16679. 

Zepeda-Moreno A, Taubert I, Hellwig I, Pietsch L, Lakshmanan VK, Wagner W, Ho AD. Innovative method for quantification of cell-cell adhesion in 96 well plates. Cell Adhesion & Migration. (2011) 5(3): 215-219.

Cholewa D, Stiehl T, Schellenberg A, Bokermann G, Joussen S, Koch C, Walenda T, Pallua N, Marciniak-Czochra A, Suschek CV, Wagner W. Expansion of Adipose Mesenchymal Stromal Cells is affected by Human Platelet Lysate and Plating Density. Cell Transplantation. (2011) 20(9): 1409-1422.

Bork S, Horn P, Castoldi M, Hellwig I, Ho AD, Wagner W. Adipogenic Differentiation of Human Mesenchymal Stromal Cells is Down-Regulated by microRNA-369-5p and Up-Regulated by microRNA-371. Journal of Cellular Physiology. (2011) 226(9): 2226-2234.

Horn P, Bork S, Wagner W. Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis.Methods in Molecular Biology. (2011) 698: 23-35.

Wagner W. Senescence is heterogeneous in mesenchymal stromal cells - kaleidoscopes for cellular aging. Cell Cycle. (2010) 9(15): 2923-2924.

Horn P*, Bokermann G*, Cholewa D, Bork S, Walenda T, Koch C, Drescher W, Hutschenreuther G, Zenke M, Ho AD, Wagner W. Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells. Cytotherapy. (2010) 12(7): 888-898.

Wagner W, Bork S, Lepperdinger G, Joussen S, Ma N, Strunk D, Koch C. How to track cellular aging of mesenchymal stromal cells?Aging. (2010) 2(4): 224-30.

Teschendorff AE, Menon U, Gentry-Maharaj A, Ramus SJ, Weisenberger DJ, Shen H, Campan M, Noushmehr H, Bell CG, Maxwell AP, Savage DA, Mueller-Holzner E, Marth C, Kocjan G, Gayther SA, Jones A, Beck S, Wagner W, Laird PW, Jacobs IJ, Widschwendter M. Age-dependent DNA methylation of genes that are suppressed in stem cells is a hallmark of cancer. Genome Res. (2010) 20(4): 440-446.

Wagner W, Ho AD, Zenke M. Different facets of aging in human mesenchymal stem cells. Tissue Engineering Part B Reviews. (2010) 16(4): 445-453.

Ong L., Li W., Oldigs J.K., Kaminski A., Gerstmayer B., Piechaczek C., Wagner W., Li R.K., Ma N. and Steinhoff G. Hypoxic/normoxic preconditioning increases endothelial differentiation potential of human bone marrow CD133+ cells. Tissue Eng Part C Methods. (2010) 16(5): 1069-1081.

Wein F, Pietsch L, Saffrich R, Wuchter P, Walenda T, Bork S, Horn P, Diehlmann A, Eckstein V, Ho AD, Wagner W. N-Cadherin is expressed on Human Hematopoietic Progenitor Cells and Mediates Interaction with Human Mesenchymal Stromal Cells. Stem Cell Research. (2010) 4(2): 129-139.

Schallmoser K*, Bartmann C*, Rohde E, Bork S, Guelly C, Obenauf AC, Reinisch A, Horn P, Ho AD, Strunk D and Wagner W. Replicative Senescence-Associated Gene Expression Changes in Mesenchymal Stromal Cells are Similar under Different Culture Conditions. Haematologica. (2010) 95(6): 867-874.

Bork S*, Pfister S*, Witt H, Horn P, Korn B, Ho AD, Wagner W. DNA Methylation Pattern Changes upon Long-Term Culture and Aging of Human Mesenchymal Stromal Cells. Aging Cell. (2010) 9(1): 54-63. 

Walenda T, Bork S, Horn P, Wein F, Saffrich R, Diehlmann A, Eckstein V, Ho AD, Wagner W. Co-Culture with Mesenchymal Stromal Cells Increases Proliferation and Maintenance of Hematopoietic Progenitor Cells. Journal of Cellular and Molecular Medicine. (2010) 14(1-2): 337-350.

Wuchter, P., Wagner, W., Eckstein, V. & Ho, A. D. (2010). Das Altern im Spiegelbild der Stammzellen - Irrwege und Auswege. In "Altern gestalten, Medizin, Technik, Umwelt". H. Häfner, K. Beyreuther, W. Schlicht (Eds). Springer eBooks. eISBN 978-3-642-14353-3. S. 69–80. https://doi.org/10.1007/978-3-642-14353-3_5.

Wuchter P, Wagner W, Ho AD. (2010). Mesenchymal stem cells - an oversimplified nomenclature for extremely heterogenous progenitors. In "Regenerative Medicine - From Protocol to Patient". Springer textbook. eISBN 978-90-481-9075-1. S. 377-395. https://doi.org/10.1007/978-90-481-9075-1_16.

Marciniak-Czochra A, Stiehl T, Wagner W. Modeling of replicative senescence in hematopoietic development.Aging. (2009) 1(8): 723-732.

Wagner W, Bork S, Horn P, Krunic D, Walenda T, Diehlmann A, Benes V, Blake J, Huber FX, Eckstein V, Boukamp P, Ho AD. Aging and replicative senescence have related effects on human stem and progenitor cells.PLoS One. (2009) 4(6):e5846. 

Cao X, Pettit ME, Conlan SL, Wagner W, Ho AD, Clare AS, Callow JA, Callow ME, Grunze M, Rosenhahn A. Resistance of Polysaccharide Coatings to Proteins, Hematopoietic Cells, and Marine Organisms. Biomacromolecules. (2009) 10(4): 907-915.

Dickhut A, Pelttari K, Janicki P, Wagner W, Eckstein V, Egermann M, Richter W. Calcification or dedifferentiation: requirement to lock mesenchymal stem cells in a desired differentiation stage. J Cellular Physiol. (2009) 219: 219-226.

Marciniak-Czochra A, Stiehl T, Ho AD, Jäger W, Wagner W. Modelling of asymmetric cell division in hematopoietic stem cells - regulation of self-renewal is most essential for efficient repopulation. Stem Cells Dev. (2009) 18(3): 377-385.

Horn P, Bork S, Diehlmann A, Walenda T, Eckstein V, Ho AD, Wagner W. Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis. Cytotherapy. (2008) 10: 676-685.

Nesselmann C, Ma N, Bieback K, Wagner W, Ho A, Konttinen YT, Zhang H, Hinescu ME, Steinhoff G. Mesenchymal stem cells and cardiac repair. Journal of Cellular and Molecular Medicine. (2008) 12: 1795-1810.

Beckermann BM, Kallifatidis G, Groth A, Frommhold D, Apel A, Mattern J, Salnikov AV, Moldenhauer G, Wagner W, Diehlmann A, Saffrich R, Schubert M, Ho AD, Giese N, Büchler MW, Friess H, Büchler P, Herr I. VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma. Br J Cancer. (2008) 19(99): 622-631. 

Wagner W, Horn P, Castoldi M, Diehlmann A, Bork S, Saffrich R, Benes V, Blake J, Pfister S, Eckstein V, Ho AD. Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS ONE. (2008) 21;3: e2213. 

Wagner W, Horn P, Bork S, Ho AD. Aging of hematopoietic stem cells is regulated by the stem cell niche.Exp Gerontol. (2008) 43(11): 974-980.

Wagner W, Saffrich R, Ho AD. The stromal activity of mesenchymal stromal cells. Transfus Med and Hemotherapy. (2008) 35: 185-193. 

Ho AD, Wagner W, Franke W. Heterogeneity of mesenchymal stem cell preparations.Cytotherapy. (2008) 10: 320-330.

Bürgers HF, Schelshorn DW, Wagner W, Kuschinsky W, Maurer MH. Acute anoxia stimulates proliferation in adult neural stem cells from the rat brain. Exp Brain Res. (2008) 188(1): 33-43.

Wagner W, Wein F, Roderburg C, Saffrich R, Diehlmann A, Eckstein V, Ho AD. Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44. Cells Tissues Organs. (2008) 188: 160-169.

Wagner W, Roderburg C, Wein F, Diehlmann A, Frankhauser M, Schubert R, Eckstein V, Ho AD. Molecular and Secretory Profiles of Human Mesenchymal Stromal Cells and Their Abilities to Maintain Primitive Hematopoietic Progenitors. Stem Cells. (2007)25(10):2638-47.

Kallifatidis G, Beckermann B, Groth A, Schubert M, Apel A, A. Khamidjanov, E. Ryschich, Wenger T, Wagner W, Diehlmann A, Saffrich R, Eckstein V, Krause U, Mattern J, Chai M, Schütz G, Ho A, Gebhard MM, Büchler MW, Friess H, Büchler P, Herr I. Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer. Cancer Gene Therapy. (2007) 15: 231-240.

Wagner W, Ho AD. Mesenchymal stem cell preparations - comparing apples and oranges.Stem Cell Rev. (2007) 3: 239-248.

Ho AD, Wagner W. The beauty of asymmetry - asymmetric divisions and self-renewal in the hematopoietic system. Current Opinion in Hematology. (2007) 14: 330-336.

Faber A, Roderburg C, Wein F, Saffrich R, Seckinger A, Horsch K, Diehlmann A, Wong D, Bridger G, Eckstein V, Ho AD, Wagner W. The many facets of SDF-1alpha, CXCR4 agonists and antagonists on hematopoietic progenitor cells. Journal of Biomedicine Biotechnology. (2007) 3: 26065-26074. 

Wagner W, Wein F, Roderburg C, Saffrich R, Faber A, Krause U, Schubert M, Benes V, Eckstein V, Maul H, and Ho AD. Adhesion of hematopoietic progenitor cells to human mesenchymal stromal cells as a model for cell-cell interaction. Experimental Hematology. (2007) 35: 314-325.

Ho, A.D., Wagner, W. (2007). Bone Marrow Niche and Leukemia. In: Wiestler, O., Haendler, B., Mumberg, D. (eds). Cancer Stem Cells - Novel Concepts and Prospects for Tumor Therapy. Springer Series on Biofilms, vol 2006/5. Springer, Berlin, Heidelberg. eISBN 978-3-540-70853-7. S. 125-139.  doi.org/10.1007/2789_2007_048.

Ho AD, Wagner W. Bone marrow niche and leukemia. Ernst Schering Found Symp Proc. (2006) 5: 125.39.

Fruehauf S., Seeger T., Maier P., Li L., Weinhardt S., Laufs S., Wagner W., Eckstein V., Bridger G., Calandra G., Wenz F., Zeller W. J., Goldschmidt H., Ho A. D. The CXCR4 antagonist AMD3100 releases a subset of G-CSF primed peripheral blood progenitor cells with specific gene expression characteristics. Experimental Hematology. (2006) 34, 1052-1059.

Wagner W., Feldmann Jr.R.E., Seckinger A, Maurer M.H., Wein F, Blake J, Krause U, Kalenka A, Bürgers H.F., Saffrich R, Wuchter P, Kuschinsky W, Ho A.D. The heterogeneity of human mesenchymal stem cell preparations - evidence from simultaneous analysis of proteomes and transcritpomes. Experimental Hematology. 2006; 34, 536-548.

Ho A.D., Wagner W. (2006). Clinical potentials of stem cells -- hype or hope? In "Stem Cell Transplantation" Zanjani E, Ho AD (Eds). Wiley Press, Weinheim. ISBN-13: 978-3-527-31018-0. S. 3-26. https://doi.org/10.1002/3527608745.ch1.

Ho AD, Wagner W, Mahlknecht U. Stem cells and ageing. The potential of stem cells to overcome age-related deteriorations of the body in regenerative medicine. EMBO Rep. (2005) 6: 35-38. 

Wagner W, Saffrich R, Wirkner U, Eckstein V, Blake J, Ansorge A, Schwager C, Wein F, Miesala K, Ansorge W, and Ho AD. Hematopoietic progenitor cells and cellular microenvironment: behavioral and molecular changes upon interaction. Stem Cells. (2005) 23: 1180-1191. 

Wagner W, Laufs S, Blake J, Schwager C, Wu X, Zeller JW, Ho AD, Fruehauf S. Retroviral integration sites correlate with expressed genes in hematopoietic stem cells. Stem Cells. (2005) 23(8):1050-8.

Wagner W, Ansorge A, Wirkner U, Eckstein V, Schwager C, Blake J, Miesala K, Selig J, Saffrich R, Ansorge W, Ho AD. Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis. Blood. 2004; 104: 675-686. Free article*. (> 120 citations)

Wirkner U, Maercker C, Abdollahi A, Wagner M, Selig J, Drzonek H, Radelof U, Kellermann A, Ansorge A, Maurer J, Schwager C, Korn B, Wagner W, Ho AD, Huber P, Ansorge W. (2004). Human genome on a chip in biomedical research. In "Molecular Diagnostics", Patrinos GP, Ansorge W. (Eds). Elsevier Science, Amsterdam, Netherlands. ISBN-13 978-0-12-546661.